How to create all possible plate layouts for an assay (4cols,4rows and 4cols,6rows)?

Fiona_Baines

I would like to use JMP to create all of the possible plate layouts for a biological assay:

 

1. where the plate has 4 cols and 4 rows and 8 samples are duplicated on the plate (example illustrated below)

2. where the plate has 4 cols and 6 rows and 12 samples are duplicated on the plate

 

Is this possible using the JMP DoE platform?

 

one example layout for a 4x4 plate with 8 duplicated samples:

 

Victor_G

Hi @Fiona_Baines,

 

I think your main design could be generated very easily with the platform "Custom DoE" :

1. Specify your 2 categorical factors "Col" and "Row" with 4 levels :
   
2. Specify a model with main effects and the 2-factors interaction. You can choose the minimum number of runs (16) to have one experiment for each of the location points like in your graph, else the recommended default number 32 will provide 2 runs for each position.

Example with the 16-runs DoE :

 

Here is the script to get to the minimum number of runs DOE :

DOE(
	Custom Design,
	{Add Response( Maximize, "Y", ., ., . ),
	Add Factor( Categorical, {"1", "2", "3", "4"}, "Row", 0 ),
	Add Factor( Categorical, {"A", "B", "C", "D"}, "Col", 0 ),
	Set Random Seed( 1171712789 ), Number of Starts( 1 ), Add Term( {1, 0} ),
	Add Term( {1, 1} ), Add Term( {2, 1} ), Add Term( {1, 1}, {2, 1} ),
	Set Sample Size( 16 ), Simulate Responses( 0 ), Save X Matrix( 0 ), Make Design,
	Set Run Order( Randomize ), Make Table}
)

Concerning the two replicates situation, you can specify in the Custom design platform the number of runs you want (higher or equal to the minimum required number, in your case 16, so you could create a 24 runs design (with 8 replicate runs) or a 32 runs design (with 12 replicate runs), but you may not have the possibility to influence where these replicate runs will be distributed.

Example with 8 duplicate runs :

Another option is to generate the design with the minimum number of runs (16), and then manually add (copy-paste) on the datatable the experiments you want in duplicate.

 

In your graph, I'm not really sure to visualize/understand where are the duplicated runs. Could you change the marker to identify more easily the runs ?

 

Hope this answer will help you,