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Looking for best DOE design

Hello all,

I am struggling to find the best DOE design for finding best conditions to my protocol.

I looked into documentation, assist to many webinar but canot manage to find the best one. So I need your help on that.

I have 2 categorical responses (4 levels), 1 continuous factor and 1 categorical (2 levels) factor.
My continuous factor is concentration of a reagent. I need to include negative control for that, meaning that I want to see the result when concentration of this factor is 0. I work on biological samples and this negative control is not an option.

I tried Custom Design but didn't get range of value for my continuous factor.

So I tried Space Filling Design but, despite giving me a large amount of tests, this design doesn't use the value 0 for my continuous factor.