Hi all,
I have made a custom DOE and have been analysing my data first step-wise to identify important effects then standard least square to find significant effects. I could see the following significant effects:

I then checked the Lack of fit, which was also ok

I then looked further down through the parameter estimates and saw a lot of red values for the parameter estimates. What does this mean for my model? Is it no longer significant even though effect summary and lack of fit says it is?

Many thanks for you help in answering this question.
Kind regards,
Hanna