Hi everyone, I would appreciate a little clarification or guidance on my experimental design.
My experiment has two main treatments: gas contamination (2 different levels) and species (2 different species). The experiment is set up so that I have two main rings (one for each level of contamination), each with the two different species in them. Each rings are replicated 3 times making it 6 rings in all to assess my parameter of interest.
I guess the basic assumption would be to test for Rings as a random effect, but I am unsure how to do this. How can I correctly estimate of the effect of the gas contamination, species, Days and interactive their effect, once I have satisfied my normality assumption?
Some guidance needed, I have added a sample excel data.
Thank you.