Hi,
I have intensities of 4 treated and 5 untreated samples taken between at a range of wavelengths (400 - 600 nm) at three different time points (0, 1 , 3 hours).
In the long run, I'm trying to normalize my data so the average intestity at 400nm of the untreated samples is 1 at each time point. In order to do that, I need to figure out how to get the average untreated, 400nm intensity at 0, 1, 3 hours.
This is my current code:
Col Mean(
:Intensity,
:Time == 1 & :Wavelength( nm ) == 400 & :Treat == "No"
)
From graphs I've made (and an Excel pivot sheet), I know the value should be about 0.72 for 1 hour, but I'm getting 1.3 for most rows. I'm also getting 0.68 for the rows that satisfy my 3 "and" statements, but I know that Excel pivot sheets sometimes do funny normalizing things, so I'm not sure if I'm concerned about the differences there.
Any suggestions for making my code consistant would be greatly appreciated.