I have been asked to analyze multiple main effects and their various interactions, but don't think I can do so as I achieve Lost DFs error messages when I use the Fit Model analysis. In case it helps, here the details of the data. Main effects (size): Location (4), Cultivar (4), Region (2, 2 Locations grouped per region), and Year (3). I only get results/appear to have enough DF when I run any two main effects maximum, but never with an interaction.
Question 1) Do I have a degree of freedom issue in terms of not being able to analyze more than two main effects, hoping to look at all four?
Question 2) If I was to run only two main effects, it is correct to rerun the model when a main effect is not found to be significant? For example, I ran Year Location and found neither to be significant, so then I reran the the model removing Year as I am interested in Location and again found Location to be non-significant, but with a lower p-value.



Appreciate any advice